Camera control software is designed based on LabVIEW 2009 in electro-optical detection system in order to control TV and infrared camera working mode and the capturing and processing of the detected images. The camera setup subprogram is designed according to the character of serial communication control, and the camera working mode is set up by sending different commanding codes. Capturing and processing subprogram are complied to realize display of video image in real time after capturing, despinning and display processing. Application results show that the camera is controlled by the program flexibly and the effect of output video images is satisfied.