Optical polarimetry is a noninvasive glucose monitoring technique which can be potentially used to ascertain blood glucose levels through measuring the aqueous humor glucose levels in the anterior chamber of the eye. One major limitation of the polarimetric approach as a means to noninvasively measure glucose levels is time-variant corneal birefringence caused by motion artifact. We design a real-time, closed-loop, dual-modulation, multi-wavelength polarimetric system for glucose measurement by using an isolated human cornea ex vivo clamped on an artificial anterior chamber. Eye coupling devices are utilized to prevent bending of the light through the anterior chamber due to index mismatch between air and aqueous humor barrier. The polarimetric setup for glucose measurement ex vivo is presented and the system predicts the glucose concentration with standard error of 18.9 mg/dL and 15.2 mg/dL in the presence of birefringence with motion at two runs. The results indicate that polarimetry can effectively be used to minimize the effect of corneal birefringence in vivo, and helps to accurately measure glucose concentration in the aqueous humor of the eye that is correlated with blood glucose levels.