As an important nonlinear optical imaging method, multiphoton excitation has demonstrated its wide application in biomedical imaging. Although shorter pulse can induce a higher nonlinear effect, most applications are still restricted in 100 fs region due to the existence of high-order dispersion. Through the compensation of high-order dispersion with multiphoton intrapulse interference, we successfully introduce 10 fs pulse into multiphoton biomedical imaging. We find that a deeper penetration and a correct excitation of multiple dyes can be obtained with this method.