Acta Optica Sinica, Volume. 32, Issue 2, 217003(2012)
Study of High Sensitive and Fast Detection to Trace Sample and Portable System
In order to carry out the goal of highly sensitive, fast and accurate detection and identification of pathogen, a novel method for highly sensitive and fast detecting trace amount of nucleic acid sample is introduced. A microfluidic chip isothermal DNA amplification for bacteria molecular diagnostics is developed. New micro-nanoliter reaction assay chip is manufactured, and the surface inertia processing technique of chip is advanced to drop the chip surface adsorption to DNA molecules. A portable confocal detector with high numerical aperture and long working distance is built to reduce background fluorescence and improve the sensitivity. The sensitivity of 5 DNA copies in micro-nanoliter reaction assay is obtained. The method and detector was verified by Escherichia coli identification to acute respiratory disease, and can meet the application of low-cost detection of clinic medicine.
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Huang Guoliang, Tian Hao, Li Zhiyong, Wang Tongzhou, Luo Xianbo, Ma Li, Wang Can, Deng Tao. Study of High Sensitive and Fast Detection to Trace Sample and Portable System[J]. Acta Optica Sinica, 2012, 32(2): 217003
Category: Medical optics and biotechnology
Received: Jul. 15, 2011
Accepted: --
Published Online: Jan. 12, 2012
The Author Email: Guoliang Huang (tshgl@tsinghua.edu.cn)