Human′s eye is a biological tissue with high scattering. Analysis on light propagation in human′s eye based on Mie theory has great significance on the research of multi-scattering imaging in biological tissue. According to the anatomical retinal structure and cellular distribution, a static structure factor from molecular dynamics is introduced to revise the radiative transfer theory, and the polarization of scattered light is numerically solved by the discrete ordinate method (DOM). The numerical results show that the polarization of scattered light depends on the cellular size and concentration distribution etc. Light polarization fluctuates for different polar angles and optical paths, and cone nucleus has more intrinsic polarization than rod nucleus. The variance of scattered light polarization increases as the scattering angle increases.