Advanced Fiber Materials, Volume. 6, Issue 5, 00438(2024)

Facile Surface Functionalization of Electrospun Elastic Nanofibers Via Initiated Chemical Vapor Deposition for Enhanced Neural Cell Adhesion and Alignment

Yerim Jang1,2、†, Soonjong Roh3,4、†, Younghak Cho1, Youngmee Jung3,7, Kangwon Lee4,5, Nakwon Choi1,2,7, Jin Yoo3,6、*, and Hyejeong Seong1,6、**
Author Affiliations
  • 1Brain Science Institute, Korea Institutes of Science and Technology (KIST), Seoul 02792, Republic of Korea
  • 2KU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul 02841, Republic of Korea
  • 3Biomaterials Research Center, Korea Institutes of Science and Technology (KIST), Seoul 02792, Republic of Korea
  • 4Department of Applied Bioengineering, Graduate School of Convergence Science and Technology, Seoul National University, Seoul 08826, Republic of Korea
  • 5Research Institute for Convergence Science, Seoul National University, Suwon-si, Gyeonggi-do 16229, Republic of Korea
  • 6Division of Bio-Medical Science and Technology, KIST School, Korea University of Science and Technology (UST), Seoul 02792, Republic of Korea
  • 7School of Electrical Electronic Engineering, YU-KIST Institute, Yonsei University, Seoul 03722, Republic of Korea
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    An advanced approach for functionalizing the surfaces of electrospun poly(l-lactide-co-ε-caprolactone) (PLCL) nanofibers for biomedical applications is presented here. Using initiated chemical vapor deposition (iCVD), a coating of the copolymer p(PFMA-co-DVB) containing poly(pentafluorophenyl methacrylate) (PFMA) and divinylbenzene (DVB) was applied to the PLCL nanofibers. This coating facilitated efficient immobilization of the biomolecules on the PLCL nanofiber surfaces, allowing precise adjustments to the polymer composition through modulation of the monomer flow rates. The resulting copolymer exhibited superior efficiency for immobilizing IgG, as confirmed by immunofluorescence intensity analysis. In vitro studies conducted with different neural cell types demonstrated that the laminin-coated iCVD-functionalized PLCL nanofibers maintained their inherent biocompatibility while significantly enhancing cell adhesion. By exploiting the elastic nature of the PLCL nanofibers, cell elongation could be successfully manipulated by controlling the nanofiber alignment, as demonstrated by scanning electron microscopy and quantification of the immunofluorescence image orientation. These findings highlight the potential of iCVD-modified PLCL nanofibers as versatile platforms for neural tissue engineering and various biomedical applications, allowing valuable biomaterial surface modifications for enhanced cellular interactions.

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    Yerim Jang, Soonjong Roh, Younghak Cho, Youngmee Jung, Kangwon Lee, Nakwon Choi, Jin Yoo, Hyejeong Seong. Facile Surface Functionalization of Electrospun Elastic Nanofibers Via Initiated Chemical Vapor Deposition for Enhanced Neural Cell Adhesion and Alignment[J]. Advanced Fiber Materials, 2024, 6(5): 00438

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    Paper Information

    Category: Research Articles

    Received: Jan. 15, 2024

    Accepted: May. 20, 2024

    Published Online: Nov. 14, 2024

    The Author Email: Yoo Jin (jyoo@kist.re.kr), Seong Hyejeong (h.seong@kist.re.kr)

    DOI:10.1007/s42765-024-00438-0

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