Porous alumina optical film with controllable pore diameter, porosity and highly ordered pore arrangement is fabricated on B207 optical glass by electron-beam evaporation and anodic oxidation. The surface morphology and transmittance of the film are examined by scanning electron microscope (SEM) and the spectrophotometer, respectively. The results indicate that the surface quality of electron-beam-evaporated aluminum film is much worse than that of electrochemically polished Al foil. A two-step anodization can improve the surface quality compared with the one-step anodization. Compared with sulfuric acid and oxalic acid solutions, porous anodic alumina film prepared by electrochemically anodizing in phosphoric acid solutions has larger pore diameter and higher porosity. The transmittance of porous anodic alumina film increases after annealing.