Acta Optica Sinica, Volume. 36, Issue 2, 211001(2016)
A 3D-PSF Selection Method for Microscopic Imaging System Based on Energy Distribution
In the image deconvolution of digital confocal microscopy system, the selection of spatial size of three dimensional point spread function (3D-PSF) is closely related to the effect of image restoration. Based on the principle of three- dimensional microscopic imaging and 3D- PSF model, the relationship between the energy distribution and the size of 3D-PSF is analyzed, and a 3D-PSF size selection method is proposed and realized, which uses the restoration efficiency - energy curve inflection point for energy threshold determination. The acquisition of image and the generation of 3D-PSF of bio-optical microscope objective of three kinds of numerical aperture or magnification are simulated, and the deconvolution restoration results of three-dimensional simulated cell images by using the 3D-PSFs with different energy are obtained. The experimental results show the effectiveness and feasibility of the proposed method, and provide a basis for automatic selection of 3D-PSF for the three-dimensional microscopic image deconvolution.
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Chen Hua, Cai Yi, Yang Fengjuan, Liang Riliu, He Shuangyan. A 3D-PSF Selection Method for Microscopic Imaging System Based on Energy Distribution[J]. Acta Optica Sinica, 2016, 36(2): 211001
Category: Imaging Systems
Received: Jun. 1, 2015
Accepted: --
Published Online: Jan. 25, 2016
The Author Email: Hua Chen (chenhua_cn@163.com)