A laser diode dual-end pumped injection-seeded Nd:YAG green laser is designed. By the reasonable design of the cavity structure parameters, the fundamental transverse mode laser is obtained because of the good match between the gain medium activation area and the fundamental transverse mode volume. In 48 hours of continuous work, the output laser which keeps good single frequency and energy fluctuation is less than 2.9%. In conductively cooled structure, the high beam quality, high repetition frequency, narrow pulse width and tunable 532 nm single-frequency green laser is implemented. The repetition frequency of the laser is 1kHz. The output energy is more than 4 mJ, and the pulse width is around 6.5 ns. The beam quality M2 of 1.166 in the horizontal direction and 1.158 in the vertical direction is detected. The output green laser with continuous tuning range of 15 GHz is achieved.