Polymer films from dopant Nolinear Optics(NLO) polymers were prepared by the spinning and coating method,then the films were poled by the electric field polarization.The EO coefficient r33 attenuation curves of poled NLO chromophore doped in polyquinoline were measured by a lock-in amplifier technology. Relying on the EO r33 experiment curves and theoretical biexponential function models, the constant items defined as the permanent EO coefficient r33 and defined as stability ratio ζ idealistically were introduced, so that the characteristic properties of EO NLO polymer were described. In addition, the attenuations of EO coefficient r33 with relaxation in 1 300 nm and 1 550 nm wavelength were discussed to observe the changes in the characterization experiments.Obtained results show that the EO coefficient r33 is the important parameters to describe the characteristic properties of these materials, and it can directly measure the EO coefficient r33 of the poled NLO polymers in selecting the NLO materials and manufacturing the wave-guide devices.