In recent years, lycopene has been paid more and more attention due to its strong antioxidant activity and various health care functions. However, lycopene extraction and determination methods are complicated and difficult to be widely applied. In this study, using spectrophotometry, petroleum ether, acetone and methanol as solvent, methylene chloride as co-solvent, starting with the absorption spectrum differences to determine the maximum absorption wavelength, then, the absorbance of lycopene standard liquid with different solubility was determined at the maximum absorption wavelength and the standard curve was established to obtain the regression equation. The effect of the amount of dichloromethane and the extraction time on the lycopene extraction efficiency was investigated, and the simple and rapid determination method of lycopene by spectrophotometry was explored. The experimental results showed that the best method for the determination of lycopene by spectrophotometry is petroleum ether as solvent, wavelength 474 nm, addition of 5 mL of dichloromethane for solubilization and extraction time of 40 min.