In order to extract more reflection characteristic information of oil film on water surface by using remote sensing technology, the authors measured and analyzed the multi-angle hyperspectral polarized reflection information of oil film based on the traditional remote sensing researches. The authors used polarization as the indicator of quantitative study, adopted a three-factor-three-level orthogonal test to analyze the incident angle, oil film thickness, band, and the influence of oil film on water surface from their interaction between each other. The results of variance analysis of this orthogonal test show that: the three factors and their interaction between each other all have an influence on the polarization of oil film; the interaction between incident angle and thickness of oil slick, and the interaction between band and thickness of oil slick both show a significant impact on polarization. That is to say, the research on oil film by using hyperspectral multi-angle polarized reflectance needs both the influence on polarization from each factor and their interaction.