In future penetration combat operations under the complex electromagnetic environment, the cooperative manned/unmanned aerial vehicles will face the problem of jamming resource allocation.To solve the problem, a method for the optimization and effectiveness evaluation of the dual-aircraft blinking jamming strategy is proposed.Under the condition of blinking jamming of cooperative manned/unmanned aerial vehicles and according to the battlefield situation, the proposed method can quickly calculate the optimal blinking period, the lateral and vertical spaces of the formation, the penetration probability and its improvement in real time, so as to evaluate the jamming performance.The simulation results show that the proposed blinking jamming strategy can greatly improve the penetration probability when the formation of manned/unmanned aerial vehicles is performing a penetration operation mission.