Journal of Innovative Optical Health Sciences, Volume. 17, Issue 5, 2342001(2024)

Effect of sodium nitroprusside on the microrheological properties of red blood cells in different media

Petr Ermolinskiy1,*... Matvey Maksimov1,**, Andrei Lugovtsov1,***, Alexey Muravyov2,****, Irina Tikhomirova3,***** and Alexander Priezzhev1,****** |Show fewer author(s)
Author Affiliations
  • 1Faculty of Physics, M.V. Lomonosov Moscow State University Moscow 119991, Russia
  • 2Faculty of Physical Education, K.D. Ushinsky Yaroslavl State Pedagogical University, Yaroslavl 150000, Russia
  • 3Faculty of Natural Sciences, K.D. Ushinsky Yaroslavl State Pedagogical University, Yaroslavl 150000, Russia
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    Red blood cell (RBC) aggregation as well as their deformation significantly affects blood microrheology. These processes depend on various factors, one of which is concentration of the nitric oxide, one of the main signaling molecule in the bloodstream. The purpose of this study was to investigate the effect of nitric oxide on the microrheological properties of red blood cells (RBCs) in RBC samples of various media after the addition of nitric oxide donor sodium nitroprusside in vitro. Microrheological properties were measured using laser aggregometer and ektacytometer based on diffuse light scattering and diffraction of laser light on a suspension of RBCs, respectively. The study found that heparin-stabilized blood showed increased RBC aggregation and deformation with sodium nitroprusside concentrations of 100, and 200μM, while EDTA-stabilized blood showed slightly decreased aggregation and unchanged deformation. With washed RBCs in dextran solution, the addition of sodium nitroprusside (in the concentrations of 100, and 200μM) resulted in decreased aggregation and increased deformation. These findings aid in our understanding of nitric oxide’s effect on RBC microrheological properties.

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    Petr Ermolinskiy, Matvey Maksimov, Andrei Lugovtsov, Alexey Muravyov, Irina Tikhomirova, Alexander Priezzhev. Effect of sodium nitroprusside on the microrheological properties of red blood cells in different media[J]. Journal of Innovative Optical Health Sciences, 2024, 17(5): 2342001

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    Paper Information

    Category: Research Articles

    Received: Jul. 28, 2023

    Accepted: Oct. 22, 2023

    Published Online: Aug. 8, 2024

    The Author Email: Ermolinskiy Petr (ermolinskiy.pb15@physics.msu.ru), Maksimov Matvey (madoway@yandex.ru), Lugovtsov Andrei (anlug1@gmail.com), Muravyov Alexey (alexei.47@mail.ru), Tikhomirova Irina (tikhom-irina@yandex.ru), Priezzhev Alexander (avp2@mail.ru)

    DOI:10.1142/S1793545823420014

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