To analyze atmospheric radiation characteristics of geostationary millimeter wave remote sensing, three different typical spectral absorption line database based line-by-line millimeter wave atmospheric absorption models, Millimeter-wave Propagation Model(MPM), ROSenkranz Model (ROS), High-resolution TRAN smission molecular absorption Model(HITRAN), were applied. Under different atmospheric temperature, pressure and water vapor conditions, the differences between the three models were analyzed on 424 GHz, which are planned to add to a geostationary satellite. A multi-layer millimeter wave radiative transfer model was presented. By utilizing intensively observing radiosonde data of Shanghai, simulated millimeter wave radiations of Fengyun 3B(FY3B) microwave humidity sounder (MWHS) were calculated under the three atmospheric absorption models. The simulations were compared with the real observations of FY3B MWHS, with temporal-spatial matching. The accuracy of the three models was analyzed. Simulations show that, for 424 GHz channels, the trends of the three models’ results are the same over atmospheric temperature, pressure and water vapor. The values of MPM and ROS are much closer to each other than HITRAN. The performance of MPM is better than ROS and HITRAN. Simulation errors in channel 5 are less than channel 3 and 4 of FY3B/MWHS.