Phenylethanolamine A (PA) is a new adrenergic agonist and can improve feed utilization and the lean meat percentage of carcasses of livestock. But the drug residue would lead to a great threaten to human, such as nausea, dizziness,limbweakness, hand tremor and other symptoms of poisoning. Long-term consumption may lead to mutations in the body’s chromosomes, induced malignant tumorsetc. At present, the main detection method is liquid chromatography-tandem mass spectrometry (LC-MS-MS). The interaction between PA and CdTe nanomaterials is rarely reported. In this study, the water-soluble CdTe nanomaterials were prepared by microwave heating method,using mercaptopropionic acid as a stabilizer. The products have good fluorescence properties. The quantum yield of the CdTe nanomaterials was measured to be 0.523 4, and the half-peak width was about 45 nm. By using CdTe nanomaterials as fluorescent probe, based on the enhancement of the fluorescence intensity of CdTe nanomaterials by PA, a simple, rapid and sensitive method for the determination of PA was proposed and validated.Furthermore,the possible mechanism of interaction between CdTe nanomaterials and PA was discussed.Effects of experimental conditions were investigated. The optimal conditions were as follows: buffer: pH 7.4 KH2PO4-Na2HPO4 solution,concentration of PA:100 μg·L-1, reaction time: 15 min, reaction temperature: 20 ℃. Under the optimal conditions, a good linearity was obtained between fluorescence intensity and PA concentration in the range of 8~120 μg·L-1 with a correlation coefficient of 0.996.The obtained linear regression equation was: F /F0=0.001 9c+1.032 1 and the limit of detection was 3.5 μg·L-1.The proposed method was successfully applied in the determination of PA in swine urine samples, at the same time, the method is compared with the traditional liquid chromatography tandem mass spectrometry. The result indicated that the combination of CdTe nanomaterials and PA produced a nice labelling effect, and this method is fast, feasible and effective.