The transmission fiber is affected by the change of ambient temperature and vibration, which will cause the phase drift of radio on fiber frequency signal, and lead to the decrease of time-frequency transmission accuracy. In order to reduce the influence of phase drift on transmission system, the optical fiber phase drift detection and phase compensation experiment verification are caried out by using the optical fiber phase-stabilized transmission technology based on fringes counting method of optical fiber interferometer. The experimental results show that the natural phase drift of 168 m transmission fiber for 28 h is 32.3 ps, and the phase drift is reduced to 1.29 fs by using piezoelectric ceramics and temperature-controlled optical fiber to compensate the natural phase.