Acta Photonica Sinica, Volume. 34, Issue 11, 1693(2005)
The Breeding of High-Yield Pectinase Producing Bacteria Using He-Ne Laser Irradiation on the Cell and Protoplasts
A mutant strain ZH-g was screened by He-Ne laser mutagenesis on the cells.Its pectinase activity was in 1.3 fold of the original one,reaching 301 μ/mL.On the basis of this,using He-Ne laser irradiating on the protoplasts of ZH-g,the mutant strain ZH-gA with high yield of pectinase was obtained.It accumulated pectinase activity up to 357 μ/mL which was 18% higher than that of the parent strain.This strain can produce pectinase efficiently under both aerobic and static cultivation.The enzyme activity is stable after several times of batch.
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[in Chinese], [in Chinese], [in Chinese], [in Chinese], [in Chinese], [in Chinese]. The Breeding of High-Yield Pectinase Producing Bacteria Using He-Ne Laser Irradiation on the Cell and Protoplasts[J]. Acta Photonica Sinica, 2005, 34(11): 1693