There is need to determination of uranium concentration at ppb level in environmental matrices. Due to low sensitivity of FAAS, UV-Visible Spectroscopy is generally used as measurement technique. In this study, ion-imprinted polymers (IIP) were prepared for uranyl ion (imprint ion) by formation of ternary (salicylaldoxime and 4-vinylpyridine) complex in 2-methoxy ethanol (porogen) following copolymerization with methacrylic acid (MAA) as a functional monomer and ethylene glycol dimethacrylate (EGDMA) as crosslinking monomer using 2,2-azobisisobutyronitrile as initiator. The synthesized polymers were characterized by FT-IR and TGA analysis. Arsenazo Ⅲ in 3 M HClO4 was used as complexing agent in the measurement step. The optimal pH for preconcentration was found to be between 3.5～6.5 values. The developed method was applied to uranium (Ⅵ) determination in natural water samples.