The polysulfone hollow fiber membranes is one of the most widely used membranes in many fields due to its unique property, microbial fouling of membrane is one of the most severe challenges duing the application of the hollow fiber membrane. Quantum dots have been widely applied to inhibit the growth of microorganisms due to its small particle size, toxicity and other characteristics. This paper studies the antibacterical properties of CdTe quantum dots modified polysulfone hollow fiber membranes. The qualified CdTe quantum dots showing narrow and symmetric emission spectrum were successfully prepared in aqueous phase, Fluorescence Spectrum (FS) and TEM measurements were employed to characterized the CdTe, and its average size was calculated to b e 3nm. Benzophennone was used as photosensitizer, the polysulfone hollow fiber membrane was prepared by continuous ultraviolet photografting polymerization of β-Hydroxyethyl methacrylate onto membrane surface. Quantum dots were successfully assembled to the surface of the membranes due to the reaction between the hydroxyl group and carboxyl group, infrared spectroscope was employed to confirmed the modified membranes.Wild stranin B1 with average size of about 10μm was isolated the and cultivated, the morphology of wild strain B1 was characterized by scanning electron microscope and polarizingmicroscope. The antimicrobial capability of Quantum dots modified polysulfone hollow fiber membrane was estimated by optical density via ultraviolet spectrophotometer. The data showed that the optical density of the aqueous bacteria was decreased from 0.88 to 0.27 after quantum dots modified memebrane was applied, and consequently, the antibacterial performance of the modified membranes was improved evidently. Results indicated that the technique of continuous ultraviolet photografting polymerization shows a promising potential for modification of hollow fiber membranes in industrial scale.