Rabi frequency and coupling strength of the Raman transition in Bose-Einstein condensate are studied experimentally. Rabi frequency is an important parameter of the interaction between light field and atoms. It is used to measure the coupling strength between light field and atoms. Raman transition coupling strength is a crucial parameter in the experiment of spin-orbit coupling. The Rabi oscillation of Raman transition between Zeeman states |1,0〉 and |1,1〉 in hyperfine state F=1 of 87Rb is investigated with different frequency detuning of Raman laser. Moreover, Rabi oscillation coupled simultaneously in the five Zeeman energy levels of hyperfine atomic state F=2 is observed with Raman laser wavelength at 800 nm. This work is conductive to optimize the experimental parameters of 87Rb spin-orbit coupling.