The FTIR (Fourier transform infrared) spectra of malignant pleomorphic adenoma tissues and surrounding normal tissues were investigated using the Spectrum GX FTIR Spectrometer. The results indicate that there were differences in the Fourier transform infrared spectra between the malignant pleomorphic adenoma tissues and the surrounding normal tissues at some bands. (a) Amide I band location related to the protein in milignant pleomorhic adenoma tissues shifted to a higher wave number compared to the normal tissues, and the absorption was increased in tumor tissues. This indicates that the degree of hydrogen-bonding herein increased; (b) The peak position related to PO2-1 group in nucleic acid shifted to a higher wave number after canceration, and the absorption was stronger in tumor tissues compared to the normal tissues; (c) The absorption of band 1 640 cm-1 related to —CH2 group in lipid was stronger but its peak position shifting was disorder.