Acta Optica Sinica, Volume. 32, Issue 2, 218001(2012)
Fast Fourier-Domain Localization Algorithm of Single Molecule with Nanometer Resolution for Super-Resolution Fluorescence Imaging
In order to improve the computational speed and prior background noise-dependent problem of single molecule localization algorithm, a Fourier domain localization scheme based on zero-padded fast Fourier transform and phase gradient operators is used to obtain a powerful mathematical model for localizing a single molecule without numerical fitting. Numerical simulations indicate that the proposed method exhibits nanometer scale localization precision while executing almost as fast as the fluoroBancroft algorithm. Furthermore, a sample consisted of several lines of molecules are simulated imaged. The results demonstrate that two lines of molecules separated by 30 nm can be resolved. Finally, super-resolution images of filopodia in HeLa cells are reconstructed based on the method, in which filopodia with diameter of 75~200 nm are resolved.
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Yu Bin, Chen Danni, Liu Lei, Qu Junle, Niu Hanben. Fast Fourier-Domain Localization Algorithm of Single Molecule with Nanometer Resolution for Super-Resolution Fluorescence Imaging[J]. Acta Optica Sinica, 2012, 32(2): 218001
Category: Microscopy
Received: Jul. 15, 2011
Accepted: --
Published Online: Dec. 16, 2011
The Author Email: Bin Yu (yubin@szu.edu.cn)