Spectrally encoded imaging uses a diffraction grating and a spectrum analyzer setup to obtain microscopic images. The different position on the sample is illuminated by different wavelength. Then the reflection light is decoded to obtain spatial information. In this letter, a spectrally encoded microscopy is described which is based on a super luminescent diode source and a home-built spectrometer. By imaging a 1951 USAF resolution target, the measured lateral resolutions were found to be 1.72±0.13 μm and 1.26±0.08 μm in the spectral and its vertical directions, respectively. The axial resolutions along the different lateral positions were measured to be unequal. The images of excised swine liver tissue were obtained at different locations. The veins, liver sinusoidal endothelial cells and hepatocytes can be visualized. The Chicken heart tissue was imaged at 10 μm per step along the depth direction beneath the surface, the images indicated the difference in the structure features at different depths. The results demonstrate high resolution, depth-resolved imaging capability by this method.