Er3+ singly doped and Er3+/Pr3+co-doped tellurite glasses 60TeO2-30WO3-10La2O3 are prepared by conventional melting method. The fluorescence spectra and lifetime of Er3+4I13/2 level pumped by 980 nm laser diode (LD) are investigated. The infrared and absorption spectra are measured. The absorption coefficient α is calculated based on the infrared spectrum. The spontaneous radiative coefficient Arad and fluorescence ratio β are acquired by Judd-Ofelt theory. Fuchtbauer-Laderburg theory is applied to calculate the stimulated emission cross section around 2.7 μm. The energy transfer processes and spectroscopic properties of Er3+-doping and Er3+/Pr3+ co-doping are analyzed under 980 nm LD excitation. The calculated absorption coefficient α of OH- is 0.57 cm-1, the spontaneous radiative coefficient Arad, fluorescence ratio β and the maximum stimulated emission cross section of Er3+4I11/2 to 4I13/2 transition are 60.6 s-1, 16.3%, and 1.13×10-20 cm2, respectively. The results indicate that this kind of tellurite glass is a promising matrix for 2.7 μm laser.