Laser & Optoelectronics Progress, Volume. 52, Issue 12, 121702(2015)
Phase Microscopy Imaging Method Based on Common-Path without Micro-Objective
Phase microscopic imaging technology has an important application significance in morphological identification as well as classification of biological cells. An interferometric imaging method is introduced, which is based on a high stability common-path interference system. In this approach, a reflecting mirror is employed to divide one beam into two parts. Then interferogram can be obtained by the overlap of these two beams. It is worth pointing out that, the interferogram can be amplified by means of geometrical magnification of the light instead of microobjective. Furthermore, the on-axis, off-axis and micro off-axis phase microscopy images can be obtained by controlling the angular position of the reflecting mirror. Then the structure of the sample can be acquired by phase retrieval. The method is suggested to be feasible by theoretical analysis and experiment result, which can provide a approach to phase microscopy imaging.
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Chen Yingzhou, Ji Ying, Xie Ming, Xu Yuanyuan, Han Hao, Bu Min, Wang Yawei. Phase Microscopy Imaging Method Based on Common-Path without Micro-Objective[J]. Laser & Optoelectronics Progress, 2015, 52(12): 121702
Category: Medical Optics and Biotechnology
Received: Jun. 16, 2015
Accepted: --
Published Online: Dec. 11, 2015
The Author Email: Yingzhou Chen (cyz622724@126.com)