Based on the two-point bending loading method and principles, the bending deformation and fatigue behavior of flexible glass were analyzed. The quantitative relationship between the bending curvature radius, bending stress, and plate spacing was derived. Employing the high-cycle fatigue theory, a stress-life (S-Nf) model for flexible glass was established. The results indicate that under two-point bending, the thickness of flexible glass specimen does not alter its bending trajectory. By adjusting the plate spacing, the desired bending curvature radius for experiment can be achieved. Neglecting the influence of flexible glass thickness, the bending curvature radius of specimen shows a linear relationship with the plate spacing, and the maximum tensile stress during bending is inversely proportional to the bending curvature radius. As the selected minimum bending curvature radius Rmin increases, the fatigue life also increases, and an increase in the maximum bending curvature radius Rmax does not significantly affect the bending fatigue cycles of specimen. The Basquin model can effectively predict the bending fatigue life of flexible glass under different alternating stresses or different bending curvature radii.