The slant column density (SCD) of ozone detected by Chinese first space-borne environmental trace gases monitoring instrument (EMI) was retrieved by using differential optical absorption spectroscopy (DOAS) method. Then the lookup table of atmospheric quality factor (AMF) was built based on SCIATRAN radiative transfer model, and the vertical column density of ozone (VCD, namely total ozone column) was finally obtained. A comparative analysis of the total ozone column from EMI, OMI and TROPOMI in the Antarctica on November 2, 2018 shows that EMI, OMI and TROPOMI all observed the high ozone values area in mid and high latitude of Antarctica (30° S～70° S) and the low ozone values area in inland Antarctica (75° S～90c S), and the correlation coefficients (R2) between EMI and OMI, TROPOMI are 0.977 and 0.958 respectively. Furthermore, the total ozone columns of EMI was compared with that of ground-based ZSL-DOAS at Great Wall station (62.22 S, 58.96 W) from October 25 to November 25, 2018, and the correlation coefficient (R2) is 0.926.