Chinese Journal of Lasers, Volume. 42, Issue 2, 215002(2015)
Detection and Analysis of Oxytetracycline Content in Egg White Using Surface Enhanced Fluorescence
With silver nanoparticles (AgNPs) as the substrate of the surface enhanced fluorescence, the OTC-Eu-AgNPs-Egg white system has a strong fluorescent characteristic, and the detection and analysis of oxytetracycline content in egg white can be realized. Three dimensional fluorescence spectra of the OTC-Eu-AgNPs-Egg white system is analyzed, and 380 nm is selected as the best excitation wavelength. Effect of the addition amount of AgNPs and Eu3+, reaction time and metal ions on fluorescence intensity is investigated, and the addition amount of AgNPs and Eu3+ is determined as 0.25 mL and 0.01 mL, respectively. A prediction model for determination of oxytetracycline content in egg white is established by using least squares support vector regression (LSSVR) between 605 nm and 630 nm, and the determination coefficient (R2) and root mean squared error of prediction (RMSEP) are 0.9909 and 0.9079 mg/L, respectively. The results show that silver nanoparticle enhanced fluorescence of europium (III) combined with LSSVR can be used to detect the oxytetracycline residue in egg white.
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Zhao Jinhui, Yuan Haichao, Hu Qi, Hong Qian, Peng Yijie, Liu Muhua. Detection and Analysis of Oxytetracycline Content in Egg White Using Surface Enhanced Fluorescence[J]. Chinese Journal of Lasers, 2015, 42(2): 215002
Category: Spectroscopy
Received: Aug. 1, 2014
Accepted: --
Published Online: Jan. 20, 2015
The Author Email: Jinhui Zhao (zjhxiaocao@sina.com)