An scanning surface plasmon resonance biosensor was constructed to quantitatively detect array samples. First, based on the optimal rotation position in plane prism-coupling mode and a double-prism detection optical path, the scanning surface plasmon resonance biosensor for array samples was constructed. Then, the sample density of array samples was calculated. Finally, by taking the distilled water and glucose solution with different concentrations in 5, 10, 15, 20, 25, 30 and 35 mg/mL as array samples to be measured, the multi-sample spots were detected by surface plasmon resonance method. Experimental results show that the resonance angles of array samples are 73.745, 73.919, 74.052, 74.185, 74.306, 74.408, 74.549 and 74.660°, respectively, and the solution concentrations and their resonance angles show a good linear relation, which proves that the proposed method and device are feasibility. The device is significant for increasing the sample density of array samples and realizing the high precision detection.