In this study the surface enhanced Raman spectroscopy (SERS) was employed to study the detection of 2-MBI. The self-assembly silver nanoparticle films on glass slides were used as SERS substrates. SERS spectra were obtained and the characteristic bands were assigned. The time and concentration dependent experiments were conducted and finally the optimized experimental conditions were confirmed to achieve the qualitative and quantitative analysis. The SESR band at 411 cm-1 was selected as the characteristic peak to do the qualitative and quantitative analysis. The results show that there was a linear correlation between the band intensity and the minus logarithm of concentration in the range of I=1 2378logc+8 3263, while the linear correlation coefficient was 0999 8. The relative standard deviation was between 0025~0084. The detection limit was 10-7 mol·L-1. It is concluded that this research has a great potential application in 2-MBI detection.