The subgrade soil scaling factor (SSSF) shows the basic properties of soil such as stiffness, gravimetry, density, and particle distribution, which are essential for disaster prediction and geotechnical engineering activities. In this paper, methods used for soil properties analysis are firstly summarized, and then a fiber Bragg grating (FBG) sensing technology is introduced. In order to acquire the properties and mechanical characteristics of soil accurately, a vibration-based method is presented, and an experiment for judging the properties of soil is conducted. As for the experiment, an FBG sensor is adhered to the upside of the vibration rod to measure its fundamental frequency. The rod vibrates freely at different-depth level of soil, and the changed data of wavelength from the FBG sensor are carefully collected. The Winkler spring model is used to analyze the relationship between the fundamental frequency and stiffness of soil. The results of this experiment suggest that data collected from FBG sensor can reflect vibration situation clearly and quantitatively. Thus the SSSF value can be calculated from the frequency-stiffness equation. The experimental results are almost identical with the theoretical derivation results. This confirms that the method presented in the paper can determine the SSSF effectively.