Spectroscopy and Spectral Analysis, Volume. 32, Issue 6, 1570(2012)
The Quantification of Gemifloxacin by Fluorescence Quenching Method Using Chitosan-Coated CdTe Quantum Dots
Chitosan-coated CdTe quantum dots can reduce QDs toxicity and enhance its stability in aqueous solution. Chitosan-coated CdTe QDs were used as fluorescence probes to determine Gemifloxacin on the basis of fluorescence quenching method. The results indicated that the relative fluorescence intensity was linearly proportional to the Gemifloxacin concentration in the range of 3.46×10-9~3.46×10-7 g·L-1 with a linear fitting equation of F0/F=1.0637+0.016 7c(g·L-1) and the RSD was 2.7%. On the basis of fluorescence quenching method theory, it was concluded that the interaction between QDs and Gemifloxacin was a kind of static quenching through hydrogen bonding and Van der Waals force, and the binding sites value was 0.8. This method with high sensitivity and broad linear range provided a new approach to determining Gemifloxacin.
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ZHONG Wen-ying, WANG Yan, HUANG Bin, SHU Chang. The Quantification of Gemifloxacin by Fluorescence Quenching Method Using Chitosan-Coated CdTe Quantum Dots[J]. Spectroscopy and Spectral Analysis, 2012, 32(6): 1570
Received: Nov. 15, 2011
Accepted: --
Published Online: Jun. 14, 2012
The Author Email: Wen-ying ZHONG (wyzhong@cpu.edu.cn)