In order to realize the quick and accurate calibration for strain and temperature response coefficients of distributed sensing optical fibers, a novel method for simultaneous calibration of strain and temperature was proposed. The temperature of sensing fiber and metal tube was controlled by the thermostat. The strain of optical fiber twined around the metal tube was controlled by the linear thermal expansion of the metal tube. The temperature change of optical fiber around the metal tube was compensated by the loose fiber in the same thermostat. So that the simultaneous, quick and accurate calibration of strain and temperature can be realized. The strain and temperature response coefficients of Brillouin frequency shift of bare single-mode fiber were calibrated using a thermostatic waterbath and a stainless steel tube. The calibration span of strain was 620 με, the calibration range of temperature was 35~75℃. The strain and temperature response coefficients of Brillouin frequency shift of bare single-mode fiber were 0.048 MHz/με and 1.06 MHz/℃ respectively. Experiment results indicate that the method can be used to calibrate the strain and temperature of minor diameter distributed sensing fiber simultanously.