A fingerprint anti-counterfeiting method was proposed. The structural information of the epidermis fingerprint, dermis fingerprint and sweat gland of the fingertip is obtained by optical coherence tomography, and the blood flow information under the fingertips of the finger is obtained by optical micro-angiography, so that the fingertip biometric feature data with dynamic anti-counterfeiting is acquired. Experiments were carried out on the effectiveness of traditional commercial fingerprint scanners and proposed method using real fingerprints and fake fingerprints. The results show that the traditional commercial fingerprint collection instrument is difficult to distinguish between true and false fingerprints, and the anti-counterfeiting ability is insufficient. The fingerprint imaging of optical coherence tomography can effectively identify common artificial fingerprint products by using the obtained subcutaneous structure of the finger, and judge the true and false fingerprint based on the fingerprint state of the dermis layer; The subcutaneous blood flow imaging obtained by optical micro-angiography can be combined with the average light intensity threshold to achieve fingerprint biopsy. The accuracy of the proposed fingerprint anti-counterfeiting method can reach 100%.