In order to find out the influence of various factors on the operational application of high-repetition-frequency laserthe principle of high-repetition-frequency laser jamming on semi-active guided missile is studiedand the influence of different factorssuch as atmospheric transmissi onrepeated frequency of jamming laser and configuration distance on the operational application of high-repetition-frequency laser is discussed respectively. The corresponding mathematical models are establishedand the simulation and calculation are conducted. The following three conclusions can be drawn from the results. 1) The higher the atmospheric visibilitythe higher the minimum peak power required by the high-repetition-frequency laser jammer; 2) The higher the jamming repetition frequencythe higher the probability of jamming success rate; and 3) The jammers shall not be configured too close or too far awayand the configuration range of the jammers should be determined according to the performance of the semi-active guided missile and the probability of jamming lead.