Selenium and selenium speciation in gingko leaf is different in different vegetal period. Each kind of dissolved selenium varies with the seasons. The total selenium content in gingko leaf picked in different season was studied and the various dissolved selenium content distribution was discussed. The selenium content in July, September and after the first frost is respectively 1.875, 2.136 and 0.815 mg·kg-1. The selenium exists in gingko leaf primarily as a water-soluble speciation. According to the experiment, the thick polysaccharide content in the gingko leaf is in the order: July