This study investigated the bioactivity and mechanical stability of hydroxyapatite (HA) ceramics under micro-vibration stress environment (MVS), and explored influence of physiological stress environment on osteoinductivity of HA ceramics. Results of scanning electron microscopy (SEM) and calcium ion release showed that MVS was beneficial for calcium ion re-deposition and bone-like apatite layer formation in HA with high micro-porosity ((29±1.2)%, (26.4±0.3)%, respectively), while calcium ion release of HA materials with a lower micro-porosity ((10.6±0.8)%) was promoted. Protein adsorption results displayed that 40 Hz MVS promoted while 60 Hz MVS significantly inhibited HA protein adsorption behavior. The compression tests demonstrated that stress environment of MVS did not affect the mechanical stability of the HA scaffolds. Our results suggested that stress environment is an important factor affecting the bioactivity and osteoinductivity of HA ceramics.