Non-uniform illumination scene is difficult to meet the single priori assumptions of existing lighting estimation method, therefore a regional-oriented non-uniform estimation method is proposed. The affecting of Lambertian object surface reflection is considered to estimate the illumination, the object surface reflectance of the homogenous regions is analyzed, and the exemplar-based support vector machine model is used to build regional illumination model of typical homogenous regions. The proposed method effectively improves the estimation accuracy about non-uniform illumination by acquiring illumination model of homogenous regions. Experimental results show that on the database of standard realistic scenes, the proposed illumination estimation method is superior to other current advanced methods.