Spectroscopy and Spectral Analysis, Volume. 32, Issue 4, 1090(2012)
Biosynthesis of CdS Quantum Dots in Saccharomyces Cerevisiae and Spectroscopic Characterization
In the present work, CdS quantum dots (QDs) were successfully biosynthesized at room temperature by using saccharomyces cerevisiae yeast as a carrier. Fluorescence emission spectra, ultraviolet-visible (UV/Vis) absorption spectra, and inverted fluorescence microscope images confirmed that saccharomyces cerevisiae can be used to biosynthesize CdS QDs. The as-prepared CdS QDs show the fluorescence emission peak at 443 nm and emit blue-green fluorescence under UV light (with excitation at 365 nm). Transmission electron microscopy (TEM) was applied to characterize the as-prepared CdS QDs and the TEM results showed that the as-prepared CdS QDs had the structure of hexagonal wurtzite. Fluorescence emission spectrum and UV/Vis absorption spectrum were used as the performance indicatiors to study the effects of saccharomyces cerevisiae yeast incubation times, reactant Cd2+ concentrations and reaction times on CdS QDs synthesis. Saccharomyces cerevisiae yeast grown in early stable phase can get the highest fluorescence intensity of CdS QDs when they were co-cultured with 0.5 mmol·L-1 of Cd2+ with 24 h incubation time. Furthermore, much more CdS QDs can be obtained by changing the culture medium during the synthesis process.
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HUANG Huai-qing, HE Ming-xin, WANG Wen-xing, LIU Jin-ling, MI Cong-cong, XU Shu-kun. Biosynthesis of CdS Quantum Dots in Saccharomyces Cerevisiae and Spectroscopic Characterization[J]. Spectroscopy and Spectral Analysis, 2012, 32(4): 1090
Received: Sep. 29, 2011
Accepted: --
Published Online: Apr. 16, 2012
The Author Email: Huai-qing HUANG (zhqhuang@dicp.ac.cn)