The data acquisition and visualization software of Mie lidar was developed based on LabVIEW. It can acquire and display data of lidar in real time by LabVIEW calling the dynamic link library of photon counting card (MSA300). The extinction coefficient was inversed by Fernald method and displayed with time-height-indication (THI) figure. The software converts and saves the data as ASCII format to be post-processed and analyzed. Lidar experiments show that the atmospheric data, extinction coefficients and THI can be displayed in real time by the software to intuitively understand the spatial and temporal changes of the aerosol and cloud.