Metallothioneins (MTs) are a family of low molecular weight,cysteine-rich and metal-binding functional proteins.Transgenic MT mushroom can be used as functional food additives,but its zinc-enriching ability has not been studied systemically until now.The zinc contents in mycelia of transgenic MT mushroom (Pleurotus ostreatus) and wild type mushroom mycelia cultivated in different zinc concentration media were analyzed by ICP-OES.The growth status,zinc-enriching ability and degree of zinc in organic form (DZOF) were also analyzed.Results showed that MT mushroom mycelia grew rapidly,but the growth was inhibited when the zinc content in solid media was higher than 1.6 mmol·L^-1.MT mushroom mycelia could enrich more zinc than that of wild type,and the zinc content in MT mushroom mycelia could be 2.56-27.49 mg·kg-1 when it was cultivated in a liquid media with 0.6-1.2 mmol·L^-1 zinc.DZOF of MT mushroom mycelia in a liquid media with 0.6 mmol·L^-1 zinc at 7 d was significantly higher (88.7%) than that in the wild type (82.1%,α=0.05),but there was no significant difference in DZOF when the MT mushroom mycelia was cultivated in a liquid media with different zinc content at 7 d.