In order to weaken the quenching effect of the metal substrate on surface enhanced fluorescence, a better fluorescent enhanced substrate was designed. In this experiment, the surface of nanoporous gold (NPG) was modified by chemical synthesis of silica, avoiding quenching effects caused by direct contact of fluorescent molecules with NPG surfaces. Rhodamine 6G (R6G) and a quantum dot with a center wavelength of 700 nm (QD 700) were assembled on the surface of SiO2@NPG respectively. The analysis of the corresponding fluorescence spectra shows that coating silica can make the surface enhanced fluorescence enhanced significantly, and the thickness of the silica has a regulatory effect on the fluorescent signal. As for quantum dots (QD), unless fluorescence enhancement of assembled QD, non-radiative energy transfer between the excited QD and NPG was observed with silica coated NPG, and the energy transfer reaches the strongest when the thickness of silica was 5 nm. The experiment provides a new method for detection based on fluorescence energy transfer.