Acta Optica Sinica, Volume. 33, Issue 12, 1211005(2013)
Hyperspectral Microscopic Imaging Based on Image Plane Interferometry
In order to obtain the spectral images of objects with small scale, a method of hyperspectral microscopic imaging based on the image plane interferometry is proposed. The interferometric imaging system with microscope objective, collimating lens, lateral shearing beam splitter, imaging lens and detector is researched. An adjustable solid structure of the lateral shearing beam splitter is adopted and rotated to achieve the interferogram of the object. The microscopic imaging principle, magnification, spatial resolution, spectral resolution and depth of field are analyzed. An experimental prototype is constructed to carry out experiments on the epidermis of broad bean leaves specimen, and the spectral images in visible band are recovered. Experimental results validate the good performance of the system with high throughput and high spectral resolution, and show that a new method for the microscopic spectral imaging is provided.
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Li Jianxin, Zhou Wei, Meng Xin, Zhu Rihong. Hyperspectral Microscopic Imaging Based on Image Plane Interferometry[J]. Acta Optica Sinica, 2013, 33(12): 1211005
Category: Imaging Systems
Received: Jun. 3, 2013
Accepted: --
Published Online: Nov. 19, 2013
The Author Email: Jianxin Li (ljx@vip.163.com)