For calibration of a recent developded spectral radiation remote sensor, a new integrating sphere radiation source with an illuminated outside laser was developed.The radiation source not only could meet the demand of the calibration for radiance responsivity,but also could scan the spectral responsivity of hyperspectral detectors accurately.A single-wavelength laser was illuminated to research the basic radiometric characteristics of the integrating sphere source.The results show that the radiance instability of the radiation source is 0.09% in half an hour,the radiance plane nonuniformity is spatially 0.16% in the central region of the sphere exit port,and the angular nonuniformity in horizontal and vertical planes within a range of ±22° are 1.8% and 0.7% respectively.These results indicate that the proposed system can calibrate directly the spectral radiance responsivity for optical instrumerts under the radiance mode,for it has advantages of higher spectral flux,lower wavelength uncertainty,and wider tunable source wavelengths.