Three different lasers with the wavelengths of 532, 632.8 and 785 nm, were used and have been given a preferable TiO2-AgNPs basement suited to each wavelength. On this condition,Raman spectra experiment of R6G and serum were conducted. When the lifting speed is up to 200 mm/min, photocatalytic activity of TiO2 thin films is no longer increased. In the range of 440~600 degrees, Titania crystal is still an anatase structure. Film thickness, temperature and ultraviolet irradiation time have an influence on the growth of silver particles together, 200 mm/min pulling speed, 520 ℃ calcination temperature and UV irradiation 80 min's condition can give TiO2-AgNPs basement a better Raman enhancement factor.It is found that the excitation laser with different wavelength has an obvious effect on spectrum.For 532 nm ,the enhancement factor of large wave number'spectral peak is significantly higher than that of small wave number,the conclusion is just opposite when laser of 785nm is used.SERSp drived by 632.8nm of the laser wavelength is different form the other two consequences and show uniform enhancement factor between large and small wave number.Paper analyzes fluorescence excitation's contribution to the Raman enhancement may be the main influence factors which lead to the SERp's significant difference .