Borosilicate bioactive glass-based (BBG) bone cement has important application prospects in the treatment of osteoporotic fractures, bone tumors, bone trauma, osteomyelitis, and other diseases due to its excellent bioactivity and biodegradability. To further understand the factors affecting its mineralization ability after implantation into human body, three kinds of amino acid were added into the conventional simulated body fluids (SBF) solution. Furthermore, to get whitlockite (WH) and hydroxyapatite (HA) simultaneously during the mineralization process, the environmental temperature, pH and Mg²⁺ concentration of SBF were adjusted. The mineralized products of BBG bone cement after being immersed in different SBF solutions were characterized. It is found that the effects of amino acids on mineralization products are quite different, of which aspartic acid and lysine affect the aspect ratio of the mineral, but glycine does not affect the morphology of the mineral. After the borosilicate bioactive glass tablet being immersed in an acidic (pH 3.5) SBF solution with a high Mg²⁺ content at 70 ℃ for a certain period, HA/WH complex mineral can be obtained.