As a kind of excellent host material for solid-state lasers, GdScO3 crystal has attracted more and more attention. In this paper, Yb,Ho∶GdScO3 crystal with the size of 30 mm×50 mm was firstly grown by Czochralski growth method. The powder diffraction data were obtained by X-ray diffraction, and the structure parameters were fitted by GSAS software. Raman spectra were measured. In the range of 100 to 700 cm-1, there are 18 Raman vibration peaks were observed. The spectral properties including transmission and emission of Yb,Ho∶GdScO3 crystal were characterized. The center of the strongest absorption band is at 975 nm, which comes from the transition of Yb3+∶2F7/2→2F5/2. The absorption cross section of Yb3+ were calculated and the values at 940, 975 nm are 0.31×10-20 and 0.42×10-20 cm2. The transition intensity parameter (Ωt) of Ho3+ was calculated by Judd-Ofelt theory, Ω4/Ω6 is 2.04. And spectral parameters such as radiation transition probability, energy level lifetime and fluorescence branching ratio were calculated. The results show that Yb,Ho∶GdScO3 is a promising candidate material for 2~3 μm laser crystals because of its excellent luminescence properties.