Spectroscopy and Spectral Analysis, Volume. 31, Issue 6, 1533(2011)
Interaction of Lactoferrin and Its Peptides with DPPC and DPPG Liposomes Studied by Raman Spectroscopy
Interaction of lactoferrin and its peptides LfcinB4-14 and LfampinB with dipalmitoylglycero-phosphocholine (DPPC) and dipalmitoylglycero-phosphoglycerol (DPPG) liposomes were studied by means of Raman spectroscopy. In our study, conformational changes in the phospholipid molecules were investigated by measuring the intensities of 2 847 and 2 882 cm-1 Raman bands which are assigned to acyl chains’ symmetric and asymmetric C—H stretching vibrations. The addition of lactoferrin and its peptides LfcinB4-14 and LfampinB caused a decrease in the 2 882 cm-1 intensity of DPPG liposomes, thus the order parameter for the lateral interactions between chains Slat decreased from 0.19 to 0.17, 0.14 and 0.12 respectively. On the contrary, the intensities at 2 847 and 2 882 cm-1 of DPPC liposomes were poorly affected by lactoferrin and its peptides. The results show that lactoferrin and its peptides present a stronger effect on the molecular structure and order degree of anionic lipid DPPG than that of zwitterionic lipid DPPC. This suggests that lactoferrin, LfcinB4-14 and LfampinB can interact and combine with the negatively charged DPPG liposomes by electrostatic interaction and perform its antibacterial activity. Besides, LfcinB4-14 and LfampinB can affect the lipid more strongly than lactoferrin.
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ZHANG Wei, REN Fa-zheng, GE Shao-yang, ZHANG Lu-da, JIANG Lu, MAO Xue-ying, GUO Hui-yuan. Interaction of Lactoferrin and Its Peptides with DPPC and DPPG Liposomes Studied by Raman Spectroscopy[J]. Spectroscopy and Spectral Analysis, 2011, 31(6): 1533
Received: Jun. 25, 2010
Accepted: --
Published Online: Jan. 5, 2012
The Author Email: Wei ZHANG (zw_7431@163.com)