Spectroscopy and Spectral Analysis, Volume. 33, Issue 8, 2128(2013)
Studies on Thermal Denaturation of Peanut Allergen Ara h1 and its Interaction with Reducing Sugars
The thermal denaturation of peanut allergen Ara h1, its interaction with reducing sugars and the corresponding changes in allergenicity were investigated by circular dichroism(CD), fluorescence and ELISA method comprehensively. The experimental results indicate that the secondary structure of Ara h1 changes significantly along with decreasing α-helical structure and its allergenicity with the temperature higher than 85 ℃, and that both xylose and fructose can stabilize Ara h1 protein structure through interacting with Ara h1 protein and decrease its allergenicity obviously. This study should be helpful to the further understanding of sensitization mechanism of food allergy and be useful for the guidance on reasonable manufacturing of peanut foods.
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XU Hong, SHEN Liang-liang, HU Zhang-li, XIAO Jie, XIAO Hua-xin, WU Jin-xia, LI Yi, WEI Bo, NI Zhuo, WU Xu-li, LIU Zhi-gang. Studies on Thermal Denaturation of Peanut Allergen Ara h1 and its Interaction with Reducing Sugars[J]. Spectroscopy and Spectral Analysis, 2013, 33(8): 2128
Received: Nov. 30, 2012
Accepted: --
Published Online: Aug. 12, 2013
The Author Email: Hong XU (xuhong@szu.edu.cn)